This forms a carbanion, which is a negatively charged carbon atom. This step can be represented as follows:
\[
\begin{array}{c}
\text{HO}^{-} + \text{R-COOC}_{2}\text{H}_{5}-\text{CH}_{2}-\text{CH}_{2}-\text{CH}_{2}-\text{CH}_{2}-\text{COOC}_{2}\text{H}_{5}
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