00:01
Our question is asking us what would happen once we use a chromo immunopurcipitation technique to isolate a dna fragment.
00:11
So we know that there are multiple forms of lab techniques used to isolate a desired product.
00:22
You know, there's different methods to isolate proteins, to isolate, dna to isolate other different molecules within the body.
00:38
And so specifically, this question is asking, how can we identify this dna fragment? so one of the important things that was also covered in our textbook and in the chapter was it talked about polly a -tales, and it talked about it also.
01:01
Talked about cytosine or cpg, right? so it talked about methylation.
01:10
So when we are talking about controlling gene expression or even just isolating a fragment of dna that we are interested in maybe manipulating to eventually activate or deactivate gene, a specific gene, we want to make sure that we get the right fragment, right? and that's what this question is asking.
01:37
So in the chapter, it also covered different post -transcriptional methods and even other methods that are used to preserve dna.
01:51
And so that's what we do.
01:52
There's several different methods we can use.
01:55
We can use acetylation.
01:56
We can use acetyl methylation or phosphorylation.
02:03
So when we acetylate, what happens is an acetyl group is added to the end terminus of a histone protein.
02:12
And another post -transcriptional modification that we can do is we can add polyatiles.
02:19
And so the polyatiles are always going to be added on the three prime end...