Question 3. (6 points total) One of the most important reactions in cellular metabolism is the
hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) and inorganic
phosphate (P) according to the reaction
ATP(aq) + H2O(l) ADP (aq) + Pâ(aq)
Using the following information from the hexokinase catalyzed phosphorylation of glucose
(Gluc) to glucose-6-phosphate (G6P) and the phosphatase catalyzed hydrolysis of G6P to Gluc
and Pi:
Gluc(aq) + ATP(aq) G6P(aq) + ADP(aq)
ÎGHK = -17.83 kJ mol-1
G6P(aq) + H2O Gluc(aq) + Pâ(aq)
AG = ?
Kp
aGluc[Gluc]eq* ap, [Pi]eq
AG6P [G6P]eq
Where a are the activity coefficients for the respective species. Useful thermodynamic data
follow:
Equilibrium concentrations and activity coefficients for G6P, Pi, and Gluc at 298.15 K and pH 7.
[G6P]eq, mol L-1 [Pi]eq, mol L-1
[Gluc]eq, mol L-š
G6P
0.0049
0.9996
api
0.9320
a Gluc
1.000
7.73x10-8
0.0045
a. (3 points) Calculate Kp.
b. (2 points) Calculate A,.Gp.
c. (1 point) Calculate A, GATP for the hydrolysis of ATP.
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