List the steps below in the polymerase chain reaction protocol in
the correct order.
1- Raise temperature again causing newly formed and original
strands to separate from each other.
2- Repeat cycle over and over again.
3- Raise the temperature to -72°C, allowing Taq polymerase to
add complementary nucleotides to the 3' end of the primers.
4- Mix DNA sample with 4 deoxyribonucleotides, an aliquot of Taq
polymerase and a large excess of 2 short synthetic DNA
oligonucleotides that act as primers.
5- Cool the sample allowing synthetic primers in mixture to bind
once again to the target DNA, which is now present at twice the
original amount.
6- Cool the mix to -60°C, allowing primers to bind to both target
DNA strands.
7- Heat mixture to -95°C to melt DNA, separating the DNA
molecules into 2 strands.
