Ligation Predictions
What would you expect to see on the plates under the following scenarios?
On each plate, indicate whether you think it would be empty (no growth), have growth in the form of
isolated colonies, or have growth in the form of a lawn across the whole plate surface.
1. If the ligation worked
+kan plate, ligation mix
2. If the ligation didn't work
+ kan plate, ligation mix
-kan plate, control mix (no DNA)
kan plate, control mix (no DNA)
+ kan plate, control mix (no DNA)
+ kan plate, control mix (no DNA)
3. If there was contamination of ligation DNA
in the no DNA sample
4. If the bacteria were killed during the
transformation process
+ kan plate, ligation mix
+ kan plate, ligation mix
-kan plate, control mix (no DNA)
-kan plate, control mix (no DNA)
+ kan plate, control mix (no DNA)
+ kan plate, control mix (no DNA)