total of nine residues:
- one of each: His, Leu, Met
- two of each: Lys, Gly, and Phe
Incubation of the native
peptide with 1-fluoro-2,4-
dinitrobenzene (FDNB) and
subsequent hydrolysis yielded
2,4-dinitrophenylhistidine.
On the other hand, treatment
of the native peptide with
cyanogen bromide (CNBr)
yielded an octapeptide and
free glycine.
Digestion of the native peptide
with trypsin gave a
pentapeptide, a tripeptide, and
free Lys. 2,4-Dinitrophenyl-
histidine was recovered from
the pentapeptide, and 2,4-
dinitrophenylphenylalanine
was recovered from the
tripeptide.
Treatment with the enzyme
pepsin produced a dipeptide, a
tripeptide, and a tetrapeptide.
The tetrapeptide was
composed of (Lys) 2, Phe, and
Gly.
What is the native sequence of
the peptide?
