2) Working in a team, develop a protocol using this template. This protocol is the pre-lab
exercise for next week, so it must be completed and approved before you leave lab today.
Team Member Name(s):
Hypothesis:
Null Hypothesis:
Dependent variable: (gel samples you will run)
I
Independent variable: (the testing causative) Choose the most appropriate for your
hypothesis.
Number of bands (one protein or multiple proteins), the position of bands (size, shape, and
charge of proteins), the darkness of bands (amount of protein), fuzziness or sharpness of bands
(purity of protein and other factors)
Positive Control:
Negative Control:
Treatments: Please describe in detail any pre-treatment of your samples that you will need to
do before running your sample, such as heating, mixing, diluting, or treating with an acid,
base, or enzyme.
