2. Environmental conditions affect protein folding.
a. High temperature and/or high pH have the following effect on protein folding: ______________
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b. An enzyme ______________ (is or is not?) able to do its job if it is exposed to high temperature or high pH because ______________ .
c. One major type of non-covalent bond that is affected by high temperature is ______________.
d. Another major type of non-covalent bond (different than that for high temperature) that is affected by large fluctuations in pH is ______________.
3. In the enzyme lab, we used a catalyst to speed up a reaction.
a. The substrate of this reaction was _____starch___ .
b. The catalyst for this reaction was _____amylose_____ .
c. The product of the reaction was _____maltose and iodine______.
d. Iodine reacts with ____substrate_____ (the catalyst, the substrate, or the product?) to produce a dark blue/black color.
4. Answer the following:
a. Before the enzyme has had time to work (at the beginning of the experiment, at time 0), the spot is ________________ (what color is the spot at time 0, containing the reaction mixture drops mixed with iodine)?
b. What does this (your answer to 5a) tell you about the contents of the tube at the beginning of the experiment? ________________
c. If the enzyme is working, the spot is ________________ (what color is the spot at time 10 minutes, containing the reaction mixture drops mixed with iodine).
d. What does this (your answer to 5c) tell you about the contents of the tube at the end of the experiment?