Numerade

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Dominador Tan

Numerade educator

In the text box below, answer the following questions. Remember to label your answers that correspond to the questions, and attempt answering all of the questions to receive full credit. 1. Use the table of the genetic code to complete the following table. The columns represent transcriptional and translational alignments. Label the 5' and 3' ends of DNA and RNA, as well as the amino (N) and carboxyl (C) ends of the protein.

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INSTANT ANSWER

9. Gene A has three possible alleles \( \left(A_{1}, A_{2}, A_{3}\right) \), gene \( B \) has four possible alleles \( \left(B_{1}-B_{4}\right) \), and gene C has five possible alleles \( \left(C_{1}-C_{5}\right) \). How many diploid genotypes are possible considering these three genes? [2 marks]

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Josee Pacheco

Numerade educator

Q2 instructions: a. Which gel shows the successful trial? For the unsuccessful trials, what problem could have occurred? In all trials: the first lane is the ladder, the next 4 lanes are control reactions, and the last 2 lanes are experimental reactions. Hard mode: try to answer a few without looking at the hints Regular mode: see the list of possible mistakes on the last page of this document b. What is the genotype of the 2 unknown individuals? H = 150bp, h = 85bp O = 100bp, ? = 225bp

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Josee Pacheco

Numerade educator

Q1 Instructions: 1. Use the stock and target concentrations from Table 1 and the indicated total reaction volume to calculate the volume of components 2-6 for a single reaction. Then, calculate the volume of water needed so that your total reaction volume matches the amount indicated in the table. 2. Multiply the single reaction volumes of components 1-6 by the number of reactions you will set up to get the final recipe for your mastermix. Notes: - The square braces, [ ], indicate a value that you need to calculate. - All your values should be calculated as if the units are µl, though you will enter only the numerical value as your answer. Keep up to 2 decimal places if needed. - Values in bold are different on each students' worksheet. Table 1 Mastermix recipe to prepare 15 PCR reactions your genotyping experiment Component | Stock concentration | Target concentration | µl for single rxn | µl for mastermix 1. dH2O | na | na | [ ] | [ ] 2. PCR buffer | 5x | 1x | [ ] | [ ] 3. MgCl2 | 25mM | 2mM | [ ] | [ ] 4. Primer mix | 20µM | 0.2µM | [ ] | [ ] 5. dNTP's | 20mM | 0.2mM | [ ] | [ ] 6. Taq polymerase* | 20 units*/µl | 1 unit | [ ] | [ ] 7. Template DNA | na | na | 2µl | (omit from mix) Total volume: 50µl * "Units" are a measure of enzyme activity. Calculate the volume of Taq stock solution to give 1 unit per reaction. This will be a different formula than for the other ingredients.

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Emily f.