Please correct me if I am wrong. I am especially confused on the diagram where I am supposed to circle protons and draw boxes surrounding the electrons. Cytochrome and a cytochrome were important in oxidative phosphorylation. He placed these macromolecules within or adjacent to his membrane, based on the knowledge at the time. Please note that the notation for some molecules was different in 1961. In order (L) Membrane Outside (R)
SH,
DPN*
FP+H*
OPNH
FPH, OH
2H
QH,
26
26
OH' J(ADP-P) H JATP
JOH
Figure 5. The original caption for this figure in Mitchell 1961 read "Diagram of chemi-omotic system for coupling phosphorylation to the oxidation of substrate SH through DPNFP tentatively identified with flavoprotein, Q tentatively identified with a quinone and the cytochromes Cy." Reprinted by permission from Macmillan Publishers Ltd. 1961 Part One B
Questions (1 point each, 5 points total)
5. Use Figure 5 above to complete the following (1 point)
a. Circle the protons.
b. Draw boxes around the electrons.
6. (1 point) In Figure 5, does a proton or an electron cross the membrane first? Proton
7. (1 point) Is the cytochrome translocating protons or electrons? Electrons
8. (1 point) In Figure 5, how many ATPs are made via the spatially positioned system? 3 ATP
