9. You are trying to separate three molecules with different sizes from a crude extract using SEC, shown below. Both molecules A and B cannot penetrate the beads, while molecule C can penetrate the beads. State in order, which molecule will be in the fraction that is collected first, second and third from the SEC. (3 marks)
A
B
C
10. Referring to Question 9, draw a schematic diagram showing the pathway taken by molecule A and C inside a glass column packed with Sephadex beads. Clearly label your diagram. (3 marks)
