nucleotides of the genetic code also direct termination of translation through three stop codons, namely UAG, UAA, and UGA.
The genetic code can be increased by engineering a tRNA and aminoacyl-tRNA synthetase pair to utilize one stop codon and Macmilli;
incorporate unnatural amino acids into proteins at specific sites (Wang et al., 2001). Expanding the genetic code and incorporating unnatural amino acids provides a powerful method for introducing novel structural, chemical, and physical properties into proteins for scientific investigation, medicine, and industry (Chin, 2011).
Using the UAG, or amber, stop codon, a laboratory in California has engineered a system to specifically incorporate
Unnatural amino acid
an unnatural amino acid named pBpa. This unnatural amino acid is capable of permanently bonding to another molecule
Native tRNA
ATRO
when exposed to UV light, in a process known as
Orthogonal
ERNA
photocrosslinking. This system could become a useful tool for discovering and defining protein interactions