Place the steps in order for performing an amylase assay at a higher temperature.
1. [ Select ] ["Quickly return the starch and amylase to the water bath", "Repeat sampling every minute", "Place starch and amylase tubes in water bath", "Mix starch and amylase, and immediately remove a t=0 sample.", "Wait about 5 minutes for the temperatures to equilibrate."]
2. [ Select ] ["Quickly return the starch and amylase to the water bath", "Repeat sampling every minute", "Place starch and amylase tubes in water bath", "Mix starch and amylase, and immediately remove a t=0 sample.", "Wait about 5 minutes for the temperatures to equilibrate."]
3. [ Select ] ["Quickly return the starch and amylase to the water bath", "Repeat sampling every minute", "Place starch and amylase tubes in water bath", "Mix starch and amylase, and immediately remove a t=0 sample.", "Wait about 5 minutes for the temperatures to equilibrate."]
4. [ Select ] ["Quickly return the starch and amylase to the water bath", "Repeat sampling every minute", "Place starch and amylase tubes in water bath", "Mix starch and amylase, and immediately remove a t=0 sample.", "Wait about 5 minutes for the temperatures to equilibrate."]
5. [ Select ] ["Quickly return the starch and amylase to the water bath", "Repeat sampling every minute", "Place starch and amylase tubes in water bath", "Mix starch and amylase, and immediately remove a t=0 sample.", "Wait about 5 minutes for the temperatures to equilibrate."]