24). Use the following information to answer questions 20-24:
As a MLT, you are responsible for making Lysis Buffer. You need to make 1 L of the
Lysis Buffer. The Standard Operating Procedure (SOP) in your lab states the Lysis
Buffer has the final concentration of the following:
0.15M Ammonium Chloride (NH4CI)
1g of Potassium bicarbonate (KHCO;) into 1L solution
0.0001M Disodium EDTA (MW = 372.24 g/mol)
Use distilled water as solute for this solution
How would you make the solution? Put the steps in order of how you would proceed.
Steps:
1) Add the appropriate amount of NH4CI, KHCO3, and Disodium EDTA into the
laboratory glassware of choice
2) Add remaining distill water until volume is 1.0L
3) Add 700 mL of distilled water to the powdered chemical.
4) Mixed the water and powdered chemicals until chemicals dissolve.
What is the correct steps?
