As PCR elongation occurs, what happens when a DNA polymerase proceeding down a template DNA strand encounters the TaqMan Probe bound to the template strand, ie. how does this allow fluorescence to be detected?
Please select your answer...
A. TaqMan probe molecules are completely degraded, allowing the reporter dye to be freed from any DNA nucleotides, and this allows fluorescence of the reporter dye
B. TaqMan probe molecules are degraded into smaller oligonucleotides, freeing the reporter dye attached to part of the probe from the quencher molecule attached to the other end of the probe, allowing probe fluorescence
C. TaqMan probe molecules are released from the template as intact oligonucleotides, keeping the quencher next to the reporter dye on the released probe
D. TaqMan probe molecules serve as primers for Taq polymerase
E. TaqMan probe molecules are incorporated into the newly synthesized DNA strands.