Calculate how many moles of A- and HA are in 0.1 M, 0.01 M, and 0.001 M buffers before and after the addition of extra HCl or NaOH, based on the initial and final pH values. Calculate the theoretical number of moles based on the concentrations and the volume of HCl/NaOH added. Use the Henderson-Hasselbalch Equation to determine the number of moles before and after the addition of HCl/NaOH.
To be used for the determined number of moles (before addition):
- For 0.1 M, pH = 6.00, pKa = 6.10.
- For 0.01 M, pH = 6.06, pKa = 6.10.
- For 0.001 M, pH = 6.14, pKa = 6.10.
To be used for the theoretical number of moles (after addition):
- For 0.1 M, pH = 6.07, pKa = 6.10, 200 microliters of 1 M NaOH added.
- For 0.01 M, pH = 7.1, pKa = 6.10, 200 microliters of 1 M NaOH added.
- For 0.001 M, pH = 2.3, pKa = 6.10, 200 microliters of 1 M HCl added.
Note: The total volume of each buffer solution is 50 mL. Show all work.