Evaluation of Antiseptics and Disinfectants
Student Name
Date
Lot Number
Record the lot number for the bacterial species used in this
investigation, which is printed on the label of the original lyophilized
culture vial.
Species
Escherichia coli
Lot number
C61680A
Observations
Activity 1
Data Table 1
Treatment
Growth
Control
0.01% Bleach
0.1% Bleach
1.0% Bleach
2.5% Lysol®
5% Lysol®
10% Lysol®
0.03% Hydrogen Peroxide
0.3% Hydrogen Peroxide
3.0% Hydrogen Peroxide
10% Isopropyl Alcohol
30% Isopropyl Alcohol
50% Isopropyl Alcohol
1. Based on your results, which antimicrobial agent(s) best
inhibited the growth of E. coli? What was the lowest effective
concentration of the agent(s)?
1. What can you conclude from your results about the relative
effectiveness of antiseptics vs. disinfectants in preventing the
growth of E. coli?