Choose all descriptions in the following list that apply to allosteric regulation.
1. Depends on concentration of effector _____
2. Requires an enzyme to activate or inactivate the protein ______
3. Uses a single enzyme for activation and inactivation of the protein ______
4. Involves non-covalent binding of regulating molecule ______
5. Involves covalent bond(s) _______
6. Is always positive; that is, having the effector or phosphate group bound to the protein activates the protein _____
7. Involves binding of something to a site different from the functional (active) site of the protein ____
8. Causes conformational changes in the protein affecting the functional (active) site____
A) 1, 2, 4, 6
B) 2, 5, 7, 8
C) 1, 3, 7, 8
D) 1, 4, 7, 8
E) 2, 5, 6, 8