A nonapeptide was determined to have the amino acid composition (Lys)2,
(Gly)2, (Phe)2, His, Leu, Met. The native peptide was incubated with 1-fluoro-
2,4-dinitrobenzene (FDNB) and then hydrolyzed; 2,4-dinitrophenylhistidine was
identified by HPLC. When the native peptide was exposed to cyanogen bromide
(CNBr), an octapeptide and free glycine were recovered. Incubation of the native
peptide with trypsin gave a pentapeptide, a tripeptide, and free Lys. 2,4-
Dinitrophenyl-histidine was recovered from the pentapeptide, and 2,4-
dinitrophenylphenylalanine was recovered from the tripeptide. Digestion with the
enzyme pepsin produced a dipeptide, a tripeptide, and a tetrapeptide. The
tetrapeptide was composed of (Lys)2, Phe, and Gly. What is the native sequence
determined to be?
His-Phe-Leu-Gly-Lys-Lys-Phe-Met-Gly
Gly-Phe-Lys-Lys-Gly-Leu-Met-Phe-His
Met-Leu-Phe-Lys-Phe-Gly-Gly-Lys-His
His-Leu-Gly-Lys-Lys-Phe-Phe-Gly-Met
☐ His-Leu-Phe-Gly-Lys-Lys-Phe-Met-Gly