During protein synthesis, one of the required steps is to “charge” a tRNA molecule with its cognate amino acid. This reaction requires ATP and is shown below: The overall reaction of amino acyl tRNA synthesis can be broken down to the following two-step sequential reactions to analyze the energetics. A. Step One: i. Provide an “arrow-pushing” mechanism for Step One of the two sequential component reactions of tRNA charging, showing which bonds are broken and which are formed during the reaction. ii. Identify the nucleophile, electrophilic center, and the leaving group in this reaction. iii. Identify the HEI(s). B. Step Two: HOMEWORK - i. Provide an “arrow-pushing” mechanism for Step Two of the two sequential component reactions of tRNA charging, showing which bonds are broken and which are formed during the reaction. ii. Identify the nucleophile, electrophilic center, and the leaving group in this reaction. iii. Identify the HEI(s). Please draw out the arrow pushing mechanisms for both questions