An E. coli culture supplemented with a mixture of hydrolyzable (e.g., lactose) and various non-hydrolyzable disaccharide sugars (e.g., sorbitol-β-galactoside, methyl-β-galactoside, sulfate-β-galactoside), under otherwise optimal growth conditions, exhibited an initial normal growth rate followed by a premature decline in growth and increased cell death.
Which of the following genetic abnormalities of the lactose operon function is the most likely cause of this unexpected result?
Which combination of oligonucleotide primers would you select for PCR-based amplification to detect this pathogen?
You have decided to clone the complete coding sequence (ORF) of the BRCA1 gene using the PCR method. Although detailed information about the gene is unavailable, partial amino acid sequences at the N-terminal portion of the protein are known.
Which of the following methods would be preferred for cloning the human BRCA1 gene?