© Macmillan Learning
One method for separating polypeptides utilizes their different solubilities. The solubility of large polypeptides in water depends upon the relative polarity of their R groups, particularly the number of ionized groups. The more ionized groups there are, the more soluble the polypeptide.
Which polypeptide, (Gly)$_{20}$ or (Lys-Ala)$_3$, is more soluble at pH 7.0?
O (Gly)$_{20}$
O (Lys-Ala)$_3$
Which polypeptide, (Glu)$_{20}$ or (Phe-Met)$_3$, is more soluble at pH 7.0?
O (Glu)$_{20}$
O (Phe-Met)$_3$