Question 2 (8 minutes)
For the reaction A + B \rightarrow C + D, catalyzed by enzyme Z, the $K_m$ for A is 0.45 mM.
Using a diagram and corresponding text, describe how you would experimentally determine the maximum
velocity of enzyme Z for substrate B. Include in your answer the concentration of substrate A you would
include in the assay mixture with reasons for your answer.
(8 minutes)