HOLLA PLEASE CAN YOU HELP ME WITH THIS? You have a plasmid that contains an ampicillin resistance gene. Your plasmid stock has a concentration of 0.2 ng/ul. You transform 10 uL of this plasmid into 100 UL of chemically competent bacteria. After heat shock, you add 390 uL of SOC recovery media. After recovery, you do not pellet your transformation and you plate 200 UL of the transformation reaction (DNA volume + competent cells + recovery media) onto LB-Amp plates. The next morning you observe 1000 colonies on your plate. Based on this information, please answer the following four questions: A. What is the transformation efficiency of your cells? Show your work: B. Suppose you plated 100 ul of competent cells that were not transformed with any DNA and you observed 100 colonies the next day on your LB-Amp plate. Provide at least two plausible explanations for this curious result. C) Describe the purpose of two positive controls that are needed for a transformation experiment.