2. Answer the following questions pertaining to Michaelis Menten equation:
a. At the beginning of the reaction, when no [S] is added, what is the value of V0?
b. What is the relationship between V0 and [S] at the beginning of the enzymecatalyzed reaction?
c. Write the full equilibrium equation of [S] + [E] [P] + [E] (note: include [ES] and
all rate constant)
d. When assuming steady state while Michaelis Menten equation is being derived,
[ES] remains constant (rate for formation = rate of dissociation). Write an
equation to describe it using the equation you wrote in question 2.c