The glycolytic enzyme, Triose phosphate isomerase, is considered a "perfect enzyme" and is able to catalyze the interconversion of glyceraldehyde-3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP), shown below, at a rate approaching the diffusion limit. The equilibrium of the reaction favors the formation of DHAP (Keq = 0.05 as written left to right), however, it is GAP that is a substrate for the next step of glycolysis. If typical intracellular concentrations of these metabolites are [DHAP] = 2 mM and [GAP] = 0.5 mM, what would be the ΔG at 25°C of this reaction as written left to right inside the cell? Hint: Start by determining the ΔG°' for the reaction as written. Then, remember the answer must be the ΔG for the reverse (GAP --> DHAP).