Which of the following explains why we choose the specific wavelengths of 260nm and 280nm in the process of determining the purity of the DNA sample?
DNA shows peak absorbance at 260 nm, while RNA peaks at 280 nm. Impurities in our DNA sample caused by excess RNA causes a high absorbance at 280 nm, reducing
the A260/A280 ratio.
Proteins shows peak absorbance at 260 nm, while DNA peaks at 280nm. Impurities in our DNA sample caused by extra proteins causes a high absorbance at 260 nm,
increasing the A260/A280 ratio.
DNA shows peak absorbance at 260 nm, while protein peaks at 280 nm. Impurities in our DNA sample caused by extra proteins causes a high absorbance at 280 nm,
reducing the A260/A280 ratio.
DNA always shows an absorbance value that is 1.8x greater at 280 nm than at 260 nm.
Protein always shows an absorbance value that is 1.8x greater at 260 nm than at 280 nm.