You want to understand your body in a more meaningful way, so over break you purify pepsin and conduct a kinetic analysis on it
2nM Pepsin
v0 (uM/sec)
300
200
100
0
20
40
60
80
[S], mM
a. (7 points) What is $k_{cat}$ for pepsin?
2nM Pepsin
1/v0 (sec/uM)
0.04
0.03
0.02
0.01
0
0
0.1
0.2
y = 0.0529x + 0.0029
0.3
1/[S] (1/mM)
0.4
0.5
0.6
b. (13 points) What concentration of substrate is present when you have 20 nM/sec velocity from 2nM enzyme?
c. (10 points) Since many people drink alcohol during the holidays, you decide to see what the effect of ethanol is on pepsin activity. Surprisingly, you find that it is a competitive inhibitor with a $K_D$ of 0.129M. If you have a blood alcohol of 0.005M, sketch new lines on the graphs above to show the effect of the competitive inhibitor on pepsin activity. Give values for the new $V_{max, app}$ and $K_{M, app}$ on your graph.