3. Why do you need to assay positive and negative control samples as well as your
experimental samples?
4. Why did you need to wash the wells after every step?
5. When you added primary antibody to the wells, what happened if your sample contained the
antigen? What if it did not contain the antigen? When you added secondary antibody to the wells,
what happened if your sample contained the antigen? What if it did not contain the antigen?
6. If you tested positive for disease exposure, did you have direct contact with one of the original
infected students? If not, what conclusions can you reach about transmissibility of disease in a
population?