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display shown?

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Jenny Wu

Numerade educator

NAME 1) Indicate which micropipettor - the P20, P200, or P1000 - should be used to set the following volumes: • 18.3 µl ? • 110 µl ? • 1 ml ? • 350 µl ? • 3.5 µl ? • 75 µl ? 2) What is the volume of the following micropipettors as they are currently set, based on the digital

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Micropipette Activity Worksheet NAME 1) Indicate which microppetior - the P2Q, P200, or P1000 - should be used to set the following volumes: [ text { + 18.3 } mu /= ] - ( 110 mu rightarrow ) - ( 1 mathrm{ml}= ) ( +350 mu 1 rightarrow ) - ( 3.5 mu= ) - ( 75 mu ) 2) What is the valume of the following micropipetors as they are curwety set, based on the digtal display thown?

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Nick Johnson

Numerade educator

What are viruses? How are they different from bacteria

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b. Draw a picture of a graph showing the temperature versus growth rate (number of microbial cells) with the minimum, optimum, and maximum growth temperatures plotted. Why would cooking the chicken properly and going beyond the maximum growth temperature for Campylobocter jejuni have prevented this patient from becoming sick?

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Jenny Wu

Numerade educator

3) How will we know if our species produces a citrate permease? When determining if a bacterial species generates citrate permease, an enzyme that permits the transfer of citrate molecules into cells, the citrate test is a useful technique in microbiology.

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Jenny Wu

Numerade educator

2) How will we know if our species produces the enzyme gelatinase? An enzyme called gelatinase is essential for the digestion of gelatin, a protein that comes from vertebrates' connective tissues. Most people are familiar with gelatin from its use in Jello and other food products. A gelatin hydrolysis test can be used to determine the presence of gelatinase, which is released by specific bacteria. 3) How will we know if our species produces a citrate permease? When determining if a bacterial species generates citrate permease, an enzyme that

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Jenny Wu

Numerade educator

Calculating Generation Time NAME__________________________ The cell numbers were measured for the THREE cells types shown below while they were growing exponentially. Use the equation from the Pre-Lab reading to determine the Generation Time for each. Show your work on the back of this page. | E. coli | | Yeast | | Mouse Fibroblast | | | :--- | :--- | :--- | :--- | :--- | :--- | | TIME (minutes) | CELLS | TIME (hours) | CELLS | TIME (days) | CELLS | | 0 | 100 | 0 | 100 | 0 | 100 | | 60 | 800 | 2 | 200 | 2 | 400 | | 120 | 6400 | 4 | 400 | 4 | 1600 | | 180 | 51200 | 6 | 800 | 6 | 6400 | | 240 | 409600 | 8 | 1600 | 8 | 25600 | Generation Time E. coli 20 min Yeast 3 hrs Mouse 1 day Graphing Results Imagine that you pick a single bacterial colony into a flask containing LB medium and start shaking at 37°C. Every 30 minutes you remove some of the culture and measure the optical density with the spectrophotometer. The results are tabulated as follows:

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Yahaira T.