--- recordings allow one to measure the current passing through an ion channel. O Whole-cell O Inside-out O Cell-attached O Outside-out
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Using patch clamp techniques voltage clamp small patch membrane that has one or a few of particular type of channel The recordings You obtain look like this channel voltage Palea" Why not (5 pts)? B) The experimental solutions used to obtain these recordings were Extracellular: 150 mM KGlutamate- is an impermeant anion 10 mM NaCl extracellular 150 mM KCl What ion(s) can carry the current observed in the figure? Explain your answer (5 pts) Draw the Current for this channel ($ pts).
Sri K.
Recently, there have been advancements in DNA and RNA acid sequence comparisons. It appears that there is a potassium channel that you would like to characterize using the patch clamp technique. Starting with the channel cDNA description, please describe the steps you would take in order to record current using an Inside-Out patch. Explain the reason for each step. Your patch clamp data is shown in the figure on the right. Plot and analyze this K-channel. Calculate the chord conductance for each of the five voltages. Does the conductance of the channel change with voltage? Is this channel rectifying? Explain why. Describe an experiment to determine whether your channel is selective for potassium ions only. Include your predicted positive and negative results. Describe any controls.
Adi S.
The figure above shows a recording from a patch-clamp experiment in which the electrical current passing across a patch of membrane is measured as a function of time. The membrane patch was plucked from the plasma membrane of a muscle cell by the technique shown in Figure $12-25$ and contains molecules of the acetylcholine receptor, which is a ligand-gated cation channel that is opened by the binding of acetylcholine to the extracellular face of the channel. To obtain a recording, acetylcholine was added to the solution inside the microelectrode. (A) Describe what you can learn about the channels from this recording. (B) How would the recording differ if acetylcholine were (i) omitted or (ii) added to the solution outside the microelectrode only?
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