00:01
Hi everyone, so the question asks us to find the correct order of the transcription and translation in eukaryotes.
00:07
But before jumping on to the correct answer or the correct order for this question, we should make ourselves familiar with the concept of the central dogma, which says dna to dna is replication, dna to rna is transcription, and rna to proteins is translation.
00:27
Also, the polymerization of amino acid in order to form the protein that is also termed as a translation and transcription is tna to rna.
00:36
So the step one of transcription would be the rna polymerase.
00:40
That is going to recognize as certain the sequence that is present on the gene and it is going to bind to that particular sequence.
00:47
And that particular sequence is nothing but the promoter sequence.
00:51
So we can write here the rna polymerase is going to bind it to the promoter.
00:57
Region of a gene.
00:59
It is going to bind to the promoter.
01:01
Okay.
01:02
Step two would be rna polymerase, it unravels the dna.
01:06
It is going to cause the unwinding of the dna and reads the template start because it has to form the rna.
01:14
That rna polymerase is dependent on the dna and from the template stand of dna or the non -coding strand of dna, it is going to form the rna.
01:24
Okay.
01:24
So step two would be rna polymerase, it unravels the dna.
01:32
It unravels dna and reads the template start.
01:37
All right? step three would be the rna polymerase, it reaches the terminator sequence.
01:43
As the name itself suggests terminator, that is going to.
01:46
That means it is going to terminate the transcription, right? so rna polymerase, it reaches the terminator sequence.
01:54
Rna polymerase reaches the terminator sequence.
01:54
Rna polymerase reaches the terminator.
01:56
Terminated sequence and it is going to terminate the transcription.
02:02
Okay? and it is going to terminate the transcription.
02:07
So it is going to stop the transcription.
02:09
All right? step 4 would be a strand of mrna is made in the 5 -dash to 3 -dash direction.
02:16
So a strand of mrn is formed in the 5 prime to 3 -prime direction in the 5 -dash to 3 -dars direction.
02:26
That means if this is the mrna, then the polarity would be 5 prime to 3 prime.
02:33
So this is the mrna.
02:35
So the template strand would be in the 3 prime to 5 prime direction.
02:40
That means it is the template strand.
02:42
Okay.
02:43
And this 5 -dash to 3 -strand would be the non -temperate strand or the coding strand.
02:50
And this template strand would be the non -coding strand.
02:54
So step 5 would be the.
02:57
Processing of mrna because we are talking about the transcription in eukaryotes.
03:03
Therefore, to prevent degradation of the mrna, it has to be processed.
03:09
It has to be processed.
03:10
It has to be processed.
03:11
So, mrna is processed and introns are removed.
03:16
Introns are basically the non -coding regions.
03:19
So they have to be removed because we only need the coding regions for the formation of the proteins.
03:25
So introns are removed with the help of the.
03:27
The splicezoom machinery and introns are basically spliced out.
03:32
So introns are spliced out...