Q3. A student is culturing one regular facultative bacterial strain using the identical medium (M9 glucose medium) at two different oxygen levels (fully aerobic and fully anaerobic). Which curve in the graph represents the cell growth at the high oxygen level (A or B) (1 pt)? What is the most possible reason to explain the growth difference in your opinion (1 pt)? Some pathogens such as Clostridium difficile are strict anaerobes. How will you culture them in lab (1 pt)? OD A B Time Q4. Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate during glycolysis. In E. coli, the gene pykA (total 1443 bp) encodes this enzyme. If pykA coding sequence (ATGTCCAGAAGGCTT...) is changed to "ATGTCCAAAAGGCTT" due to a spontaneous mutation during DNA replication, what will happen to PykA protein? (2 pts). Q5. What are DNA sequences involved in the initiation and termination of bacterial transcription and translation (2 pts)?
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In aerobic conditions, these bacteria typically grow faster and reach a higher cell density because aerobic respiration generates more ATP than fermentation or anaerobic respiration. - Therefore, the curve that shows a higher growth rate and possibly a higher Show more…
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