Q4.8. The figure below shows a snippet of DNA in the process of being replicated. The RNA primer is shown as red while DNA is black. What will happen next? (Hint: Recall the order in which you used enzymes in the DNA replication simulation in Section 2.) 5' 3' CGTAGATCCGTACGCCT GCAUCUAGGCATGCGGA 3' nick 5' Ligase will seal the nick in the bottom strand. DNA polymerase I will replace the primer with DNA. Helicase will separate the strands. DNA polymerase III will extend the primer.
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Refer to the figure below. What bases will be added to the primer as DNA replication proceeds? The bases should appear in the new strand in the order that they will be added starting at the end of the primer.
Arun B.
Please refer to the videos on DNA replication or a reputable internet source (.edu, .org, .net, .gov) to match the proteins or enzymes with their role in DNA replication. DNA Helicase: unwinds the DNA and separates the two strands. Single-Strand Binding Proteins (SSBs): adds new RNA nucleotides to the DNA strand. DNA Polymerase: adds new DNA to the lagging strand only. Primase: replaces the RNA nucleotides in the primer with DNA nucleotides.
Madhur L.
Consider the following segment of DNA, which is part of a much longer molecule constituting a chromosome: $$5^{\prime} \ldots . ATTCGTACGATCGACTGACTGACAGTC....3'$$ $$3^{\prime} \ldots . TAAGCATGCTAGCTGACTGACTGTCAG....5'$$ If the DNA polymerase starts replicating this segment from the right, a. which will be the template for the leading strand? b. Draw the molecule when the DNA polymerase is halfway along this segment. c. Draw the two complete daughter molecules. d. Is your diagram in part $b$ compatible with bidirectional replication from a single origin, the usual mode of replication?
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