Laboratory Exercise 13: Bacterial Isolation and Identification
Student Name
You are given a mixed culture of two organisms in a small tube of TSB. These two organisms include a Gram-negative strain (your bacterial strain to identify) and a Gram-positive strain (a contaminant). Your first task is to isolate these organisms, which will help you identify which Gram-negative strain you are working with.
You inoculate two streak plates on TSA media and MacConkey Agar using aseptic technique. For each medium, state whether the medium is selective, differential, or neither and explain if this medium will help you isolate your Gram-negative organism as pure culture.
TSA -