1) A cis test involving 2 T4 rII mutant phage yields 200 plaques on E. coli B and 5 plaques on E. coli K12. The bacterial lysate from the initial coinfection was diluted 10X before addition to E. coli B in the final assay and was added without dilution to E. coli K12. What is the map distance between these two mutations? a. 25 mu b. 10 mu c. 0.25 mu d. 0.5 mu e. 5 mu 2) Based on the following deletion map, where A, B and C represent deletions and 1-4 represent point mutations of the rII locus of the T4 phage, which of the following statements are TRUE? a. None of the recombinant products of the coinfection of 3 and 4, will be able to generate plaques on E. coli K12 strain b. Some of the recombinant products of the coinfection of B and C will be able to generate plaques on E. coli K12 strain c. All of the statements are FALSE d. None of the recombinant products of the coinfection of A and B, will be able to generate plaques on E. coli K12 strain e. Some of the recombinant products of the coinfection of A and 3 will be able to generate plaques on E. coli K12 strain
Added by Johnny J.
Close
Step 1
coli K12 (5 plaques) by 2 and then dividing by the total number of plaques on E. coli B (200 plaques). Show more…
Show all steps
Your feedback will help us improve your experience
Dominador Tan and 61 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
E. coli strain B (permissive) were co-infected with rlla and rllb mutant phage T4. Progeny were plated in a dilution series with the following results: Lawn Dilution Number of plaques Strain B 10^-6 10 Strain K 10^-4 5 What is the recombination frequency? Remember that the readout is viability and you cannot see the inviable progeny.
Josee P.
Strain B E. coli cells were co-infected with rII mutants X and Y. Progeny phage plated in a dilution series on both E. coli B and K gave the following results: - Number of plaques on Bacterial Lawn of Strain B: 10^-8 - Number of plaques on Bacterial Lawn of Strain K: 10^-4 What is the recombination frequency between the sites of mutants X and Y? Make sure to account for the dilution difference between the two strains (Hint: see Appendix 1).
Sri K.
A geneticist is working with a new bacteriophage called phage Y3 that infects $E$ coli. He has isolated eight mutant phages that fail to produce plaques when grown on $E$. coli strain K. To determine whether these mutations occur at the same functional gene, he simultaneously infects $E .$ coli $\mathrm{K}$ cells with paired combinations of the mutants and looks to see whether plaques are formed. He obtains the following results. (A plus sign means that plaques were formed on $E$ coli $\mathrm{K}$; a minus sign means that no plaques were formed on $E$ coli K.) (TABLE CAN'T COPY) a. To how many functional genes (cistrons) do these mutations belong? b. Which mutations belong to the same functional gene?
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
Watch the video solution with this free unlock.
EMAIL
PASSWORD