1. BATCH CULTURE TIME. Zymomonas mobilis is used to convert glucose to ethanol in a batch fermenter under anaerobic conditions. The yield of biomass from substrate is 0.06g/g, $Y_{PX}$ is 7.7g/g. The maintenance coefficient is 2.2g/(g*h), the specific rate of product formation due to maintenance is 1.1 h¯¹. The maximum specific growth rate of Z. mobilis is approximately 0.3 h¯¹. Five grams of bacteria are inoculated into 50 liters of medium containing 12g/L glucose. Assume net specific growth rate equals maximum specific growth rate and kd=0, determine the batch culture times required to
1) Achieve 90% substrate conversion
2) Produce 100g of ethanol