c) Zymomonas mobilis is used to convert glucose to ethanol in a batch fermenter under anaerobic conditions. The yield of biomass from substrate is 0.05 g/g; YPX is 7.7 g/g. The maintenance coefficient is 2.2 g/g-h; specific rate of product formation due to maintenance is 1.1/h. The maximum specific growth rate of Z. mobilis approximately 7.2/day. 10 g bacteria are inoculated into 100 liters of medium containing 12 g/l glucose. Determine batch culture times required to: i) Produce 30 g biomass ii) Achieve 90% substrate conversion iii) Produce 0.5% (w/v) ethanol
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To produce 30 g of biomass: The yield of biomass from the substrate is 0.05 g/g. This means that for every gram of glucose, 0.05 g of biomass is produced. To produce 30 g of biomass, we would need 30/0.05 = 600 g of glucose. Given that the medium contains 12 g/L Show more…
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