00:01
Here we have been asked to describe the process of dna replication.
00:09
So if we start the process of dna replication, then we can tell that it is the biological process in which two identical copies of dna are produced from one original dna molecule and the steps involved for this replication they are as follows.
00:50
The first step in the dna replication is to unzip the double helix structure of dna molecule and this is carried out by an enzyme which is known as helicase.
01:23
Next is this helicase also breaks the hydrogen bonds which are holding the complementary basis of dna together.
01:39
Next is the separation of two single strands of dna which creates a y -shaped structure and that is known as the replication fork.
02:12
Now the two separated strand will act as the template for making the new strands of dna.
02:18
And one of the strand is oriented into the 3 prime to 5 prime direction towards the replication fork.
02:29
And this is known as the leading strand.
02:34
Another strand is oriented in the 5 prime 2 -3 prime direction which is away from the replication fork and they are known as the lagging strand.
02:53
Now as a result of their different orientation, the two strands are replicated differently and a short piece of rna which is called as the primer.
03:15
This primer is produced by an enzyme known as primase and it comes along and binds to the end of leading strand.
03:28
Now the primer acts as the starting point for dna synthesis and the dna polymeris bind to the leading strand and then can tell that dna polymerase also comes into play which goes along with the replication and the primer which was acting as the starting point it also starts its work now as it walk along that is dna polymerase goes along with the leading strand it is adding new complementary nucleotide basis to the strand of dna in the 5 prime to 3 prime direction and this replication is continuous.
04:39
So we can tell that the 5 prime to 3 prime direction replication which occurs in this is a continuous process and numerous rna primers they bind to the primase enzyme and bind at various points along the lagging strand.
04:59
Now chunks of dna which are known as okazaki fragments...