In the fully automated Sanger method of DNA sequencing, all four chain-terminating ddNTPs are added into a single reaction mix. This is different from the traditional Sanger method of DNA sequencing, where a different reaction mix is prepared for each ddNTP. Identify the most appropriate explanation for mixing all four ddNTPs in a single reaction mix in the automated method of DNA sequencing.
A) The fully automated Sanger sequencing reaction mix does not contain any dNTPs.
B) The fully automated Sanger sequencing reactions do not require DNA polymerase because the bases are read directly as DNA is pulled through a tiny pore at the end of a capillary gel.
C) The fully automated Sanger sequencing reactions generate a set of DNA strands, each of which carries a single fluorescent tag whose color reveals the identity of the base that is at the end of the DNA product.
D) The fully automated Sanger sequencing reactions utilize all four ddNTPs in a single reaction mix, and all four ddNTPs are incorporated in every newly synthesized strand of DNA.