Unknown Bacteria Subculture each unknown on a BHIA slant MacConkey Agar Plate No Growth MacConkey agar Plate Growth Gram Positive Cocci Gram Negative Rods Catalase Test Lactose Non-fermenter Catalase Positive (Staphylococcus sp.) Catalase Negative (Streptococcus sp.) Table 3 Species TSI Reactions Urea Glucose Lactose Sucrose H2S Broth Proteus vulgaris Acid and Gas Negative Positive Positive Positive Serratia marcescens Acid and Gas Negative Negative Negative Negative Table 1 Staphylococcus sp. Coagulase Mannitol Blood Agar S. aureus Positive Acid Beta Hemolysis S. epidermidis Negative No Acid Gamma Hemolysis Lactose Fermenter Streptococcus sp. 6.5% NaCl BHIA Plate (45%C) Blood agar Plate Bacitracin Hemolysis S. Pyogenes No Growth No Growth Sensitive Beta S. lactis No Growth No Growth Resistant Gamma S. faecalis Growth Growth Resistant Gamma Table 2 Table 4 Species Indole Methyl Red VP Citrate Urea broth Motility Escherichia coli Positive Positive Negative Negative Negative Negative or Weak Positive Enterobacter cloacae Negative Negative Positive Positive Negative Positive Klebsiella pneumoniae Negative Positive Negative Positive Positive Negative
Added by Fernando T.
Close
Step 1
Step 1: Subculture the unknown bacteria on BHIA and MacConkey Agar plates. Show more…
Show all steps
Your feedback will help us improve your experience
Josee Pacheco and 101 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Unknown bacteria identification. Find genus and species of unknown bacteria. You can use at least 3 species to narrow it down. Given Information: Cell size - 0.8 * 2.7 micrometers Cell shape - straight rod Cell arrangement - chains, pairs, single cells Gram reaction - Purple (gram positive) Motility - Brownian motion only Endospore - stains pink Inclusion bodies - Absent Sporangium - diameter of spore is equal to a vegetative cell or absent Water-soluble pigments - No Colony pigmentation - No pigment (White or cream) Odor from the plate - none identified Oxidative/fermentation glucose with oil - yellow color Oxidative/fermentation glucose without oil - yellow color Temperature growth range - 30-37 degrees Celsius Thioglycolate broth tube - growth on the top and growth on the bottom MacConkey agar - no growth Glucose utilization - positive Sucrose - positive Maltose - negative Xylose - negative Mannitol - positive Lactose - positive Hemolysis - agar is weakly greening around colonies (alpha hemolysis) Oxidase - brown color on the filter (negative) Catalase - no bubbles produced (negative) Gelatinase - media is stiff after chilling (negative) Nitratase - media remains colorless (negative for nitrate test) Citrase - slant is green (negative) Find the genus and species.
Keemin L.
1. Can the standard plate count method be used for the enumeration of specific bacteria on selective and differential media? Yes or no. 2. The selective agent used in the EMB agar is ________. Multiple choice: a) the combination of dyes (Eosin Y and methylene blue) b) the presence of bile salts c) the sugar lactose d) the high concentration of NaCl Why is the presence of E. coli significant in an EMB test? Multiple choice: a) Rapid lactose fermentation indicates pathogenicity. b) The presence of E. coli can indicate human waste contamination. c) E. coli is used as a control organism to ensure the test is working properly. d) E. coli is always a pathogenic organism. 4. EMB tests differentiate between ________. Multiple choice: a) rapid and slow fermentation of lactose by gram-positive bacteria b) rapid, slow, and non-fermentation of lactose by gram-negative enteric bacteria c) positive and negative fermentation of lactose by gram-negative bacteria d) positive and negative fermentation of glucose by gram-negative bacteria 5. The EMB test is used most often for environmental testing. True or False. 6. The differential agent in the EMB test is ________. Multiple choice: a) lactose b) Eosin Y c) methylene blue d) glucose 7. The absorbance of light is measured with a spectrophotometer, and the result can be used to estimate the number of cells in a broth culture. True or False. 8. Which of the following types of media would be appropriate to use in order to culture a fastidious organism? Check All That Apply: a) Inorganic salt broth b) Yeast extract agar c) Nutrient agar d) Glucose salt broth
Sri K.
The Gram-negative bacterium Yersinia pestis, the causative agent of the plague, is extremely virulent. Upon infection, $Y$. pestis injects a set of effector proteins into macrophages that suppresses their phagocytic behavior and also interferes with their innate immune responses. One of the effector proteins, YopJ, acetylates serines and threonines on various MAP kinases, including the MAP kinase kinase kinase TAK1, which controls a key signaling step in the innate immune response pathway. To determine how YopJ interferes with TAK1, you transfect human cells with active YopJ (YopJ $^{\mathrm{WT}}$ ) or inactive YopJ (Yop] $^{\mathrm{CA}}$ ) and with FLAG-tagged active TAKI (TAK1WT) or inactive $TAKI (TAK1$ $^{K 63 W}$ ), and assay for total TAKI and for phosphorylated TAK1, using antibodies against the FLAG tag or against phosphorylated TAK1 (Figure $Q 23-1$ ). How does YopJ block the TAK1 signaling pathway? How do you suppose the serine/threonine acetylase activity of YopJ might interfere with TAK1 activation?
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD