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1) A thorough understanding of the process of PCR will help in the analysis of your DNA gel. The figure below represents a segment of double-stranded DNA with 100 base pair segments denoted by each letter. The primers are indicated by the arrows for both the sense and anti-sense DNA strands. In the following questions, a "copy" of DNA refers to a double-stranded piece of DNA. 5' A B C D E F G H I 5' --------------------------------- 3' 3' --------------------------------- 5' A B C D E F G H I 5' A. Draw the first three cycles of PCR indicating the intermediate products labeled with letter designations, directionality (5' ? 3'), and the size of the desired target double-stranded DNA product. Include the temperatures and their significance for each step in the PCR reaction for the first cycle. (9 points, 3 pts per cycle) B. For each cycle, indicate how many copies of target double stranded-DNA and "intermediate DNA" (the DNA includes target DNA region plus a bit of the flanking DNA and/or the original DNA strand) you would have after each cycle assuming you began with one double strand DNA template. (6 points, 2 pts per cycle)

          1) A thorough understanding of the process of PCR will help in the analysis of your DNA gel. The figure below represents a segment of double-stranded DNA with 100 base pair segments denoted by each letter. The primers are indicated by the arrows for both the sense and anti-sense DNA strands. In the following questions, a "copy" of DNA refers to a double-stranded piece of DNA.

        5'
A B C D E F G H I
5' --------------------------------- 3'
3' --------------------------------- 5'
A B C D E F G H I
        5'

A. Draw the first three cycles of PCR indicating the intermediate products labeled with letter designations, directionality (5' ? 3'), and the size of the desired target double-stranded DNA product. Include the temperatures and their significance for each step in the PCR reaction for the first cycle. (9 points, 3 pts per cycle)
B. For each cycle, indicate how many copies of target double stranded-DNA and "intermediate DNA" (the DNA includes target DNA region plus a bit of the flanking DNA and/or the original DNA strand) you would have after each cycle assuming you began with one double strand DNA template. (6 points, 2 pts per cycle)

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1) A thorough understanding of the process of PCR will help in the analysis of your DNA gel. The figure below represents a segment of double-stranded DNA with 100 base pair segments denoted by each letter. The primers are indicated by the arrows for both the sense and anti-sense DNA strands. In the following questions, a "copy" of DNA refers to a double-stranded piece of DNA.

5'
A B C D E F G H I
5' ——————————— 3'
3' ——————————— 5'
A B C D E F G H I
5'

A. Draw the first three cycles of PCR indicating the intermediate products labeled with letter designations, directionality (5' ? 3'), and the size of the desired target double-stranded DNA product. Include the temperatures and their significance for each step in the PCR reaction for the first cycle. (9 points, 3 pts per cycle)
B. For each cycle, indicate how many copies of target double stranded-DNA and "intermediate DNA" (the DNA includes target DNA region plus a bit of the flanking DNA and/or the original DNA strand) you would have after each cycle assuming you began with one double strand DNA template. (6 points, 2 pts per cycle)

Added by Jordan S.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

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Solved by Expert Farhan Anwar

09/25/2022

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A thorough understanding of the process of PCR will help in the analysis of your DNA gel. The figure below represents a segment of double-stranded DNA with 100 base pair segments denoted by each letter. The primers are indicated by the arrows for both the sense and anti-sense DNA strands. In the following questions, a "copy" of DNA refers to a double-stranded piece of DNA. A. Draw the first three cycles of PCR indicating the intermediate products labeled with letter designations, directionality (5' → 3'), and the size of the desired target double-stranded DNA product. Include the temperatures and their significance for each step in the PCR reaction for the first cycle. (9 points, 3 pts per cycle) B. For each cycle, indicate how many copies of target double stranded-DNA and "intermediate DNA" (the DNA includes target DNA region plus a bit of the flanking DNA and/or the original DNA strand) you would have after each cycle assuming you began with one double strand DNA template. (6 points, 2 pts per cycle)

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00:09 Okay, so we first need to draw the first three cycles of pcr indicating intermediate products labeled with the letter, designations, directionality, etc.

00:18 So, first intermediate product is going to be h all the way through to a.

00:37 So this is going to be the first round of the...

00:56 Okay, so i basically kind of just jumped ahead to save some time.

01:00 So the first pcr reaction i've shown in black.

01:03 The second pcr reaction are the intermediate products in blue, or i'm sorry, in red, and the third pcr reaction is shown here in blue.

01:13 What i'm showing, with the red and the black, is basically where the primers will bind to create that product from the second pcr reaction.

01:22 And then in the second pcr reaction, the blue is indicating where the primers would bind.

01:27 So by the third pcr reaction, we should have our completed product.

01:30 Okay? okay.

01:32 Okay, that's a.

01:39 Include the temperatures and the size of the desired target double -stranded dna.

01:46 Well, you have all that.

01:47 Include the temperatures and their significance for each step in the pcr reaction for the first cycle.

01:52 All right, what a convoluted question.

01:54 All right.

01:55 For the temperatures, let's go over here real quick...

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