1. To genetically transform an entire organism, you must insert the new gene(s) into every cell in the organism. Which organism is better suited for total genetic transformation–one composed of many cells, or one composed of a single cell?
2. Describe how you could use two LB nutrient agar plates, some E. coli, and some ampicillin,to determine how E. coli cells are affected by ampicillin.
3. What would you expect your experimental results to indicate about the effect of ampicillin on t. coli cells?
4. On which of the plates would you expect to find bacteria most like the original untransformed E. coli colonies you initially observed? Explain your prediction.
5. If there are any genetically transformed bacterial cells, on which plate(s) would they most likely be located? Explain your prediction.
6. Which plates should be compared to determine if any genetic transformation has occurred? Why?
7. What is meant by a control plate? What purpose does a control serve?