00:01
Hello everyone, here in this question two statements are given and we have to state whether these statements are true or false.
00:07
Now these statements are regarding the process of gram staining.
00:14
Okay, so let us first understand what is this gram staining.
00:18
Now the principle of gram staining is the ability of the bacterial cell wall to retain the crystal.
00:37
Violet dye during the solvent treatment.
00:46
Okay.
00:47
Now there are four steps which are involved in this procedure.
00:51
The first step is the initial staining.
01:00
The second step is fixing the dye.
01:07
The third step is treatment with the decolorizer which is the solvent treatment and fourth is the counter stain treatment okay now initial staining it is done with crystal voilid fixing the dye is done with the help of iodine decolorizing is done with the mixed solvent ethanol plus acetone and the counter stain it is done with the help of saffronin or basic fucin.
01:59
Okay.
02:00
Now let us see further how this process takes place.
02:04
Okay.
02:05
Now we have two bacteria, gram positive bacteria and the gram negative bacteria.
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Now in case of gram positive bacteria, the peptidoglycan, the peptidoglycan, it is high, whereas in negative it is low.
02:30
In case of gram positive, the lipid content is low, whereas in negative it is high.
02:40
So, hence, when the first step is done, that is the initial staining, which is done with the help of crystal violet, what happens is in gram positive the crystal violet color is retained.
03:02
In case of negative, it does not retain.
03:09
Okay? now, hence, the color which we will see under the microscope would be purple brown for positive and for negative.
03:22
It would be the color red.
03:28
Okay.
03:29
Now next we will fix the dye...