1. What is the dilution if 0.1 mL of broth is transferred to 4.5 mL of water? 2. If the dilution is the same in the previous question, you have made 1 plate, and you know that there are 368 colony-forming units per mL in your agar pour, how many colonies would you expect to see on your pour plate? 3. Reference to the previous question. Should you select the plate that you made if you needed to calculate the number of colony-forming units per mL in your original sample? Why or why not?
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Farhan A.
3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways that you could dilute a sample to make a 10-9 dilution? 5. If you count 198 colonies on your 10-4 tube dilution, how many bacteria/ml were in your sample? 6. You pipette 1 ml of your sample into a 9 ml dilution blank, and then pipette 1 ml of that dilution into a 99 ml dilution blank. Finally you pipette 0.1 ml of that last dilution into a plate. What is the dilution of that plate?
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"In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals." This statement describes which of the following? a. broth culture b. pour plate c. streak plate d. dilution plate
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