1. What is the relationship between the type of mutation (substitution/insertion/deletion) and HDR efficiency? 2. To what extent does the size of insertion or deletion influence HDR efficiency with small (30-60 bp) homologous arms? 3. Are mutations introduced to the gRNA binding site or PAM site best for reducing recutting?

Name: 1 what is the relationship between the type of mutation substitutioninsertiondeletion and hdr efficiency 2 to what extent does the size of insertion or deletion influence hdr efficiency with 45426
Uploaded: 2023-07-31T19:54:13-08:00
Duration: 1 min 55 s
Channel: Dave Kratz
Description: 1 what is the relationship between the type of mutation substitutioninsertiondeletion and hdr efficiency 2 to what extent does the size of insertion or deletion influence hdr efficiency with 45426

          1. What is the relationship between the type of mutation (substitution/insertion/deletion) and HDR efficiency?
2. To what extent does the size of insertion or deletion influence HDR efficiency with small (30-60 bp) homologous arms?
3. Are mutations introduced to the gRNA binding site or PAM site best for reducing recutting?

Added by Shari O.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Dave Kratz

07/31/2023

Step 1

Substitutions are generally more efficient than insertions or deletions in promoting HDR. Show more…

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1. What is the relationship between the type of mutation (substitution/insertion/deletion) and HDR efficiency? 2. To what extent does the size of insertion or deletion influence HDR efficiency with small (30-60 bp) homologous arms? 3. Are mutations introduced to the gRNA binding site or PAM site best for reducing recutting?