1-What microbial characteristics can one ascertain from a simple stain? 2-A student is directed to make a simple stain of E. coli with crystal violet, but got mixed up and used safranin instead. How would their observations be different? Would the information obtained be any different? 3-How is the procedure different when taking cells from a solid medium compared to taking cells from a liquid medium? Why is it important that your smear be thin?
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From a simple stain, one can ascertain the following microbial characteristics: - **Cell shape**: cocci (spherical), bacilli (rod-shaped), or spirilla (spiral-shaped) - **Cell arrangement**: single cells, pairs, chains, clusters, or other arrangements - **Cell Show more…
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1. A student is directed to make a simple stain of coli with crystal violet, but got mixed up and used safranin instead. How would their observations be different? Would the information obtained be any different? 2. How is the procedure different when taking cells from a solid medium compared to taking cells from a liquid medium? Why is it important that your smear be thin?
Rabeya Z.
2. List and describe the types of staining techniques that exist in microbiology. Which type of stain would be best to use to identify the presence of a Gram negative bacteria in an unknown microbial sample? Explain your answer. 3. Draw a diagram of a prokaryotic cell. Label the major cell parts and describe one function of each part. 4. Draw the process of binary fission and the bacterial growth curve. Explain what is happening in each process. In what stage of the bacterial growth curve would the most binary fission be occurring? 5. Explain the different types of growth media that are used to culture microorganisms. How could using different growth media help in isolation of a single bacterial species?
Sana R.
1) Why are spores so difficult to stain using common staining methods? 2) What differences can you observe between the two smears of Bacillus 24 after 24 hours and Bacillus after 48 hours? Include differences in cell morphology, color of vegetative cells, relative number of spores, and any other obvious differences.
Nicholas M.
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