You have isolated a population of cells from the inner cell mass of a mouse embryo. You suspect these cells are germ cells and want to verify if they have pluripotent stem cell potential. Propose an experiment that demonstrates the stem-like potential of the germ cells. Explain the attributes that meet and don't meet the requirements of a pluripotent stem cell expected at the end of your experiment.
Added by Cody C.
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First, we need to culture the isolated germ cells in vitro. We can use a feeder layer of mouse embryonic fibroblasts (MEFs) or a defined culture medium that supports the growth of pluripotent stem cells. Show more…
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