1. You inoculate a species of bacteria on a MacConkey agar which normally shows positive for growth and ability to breakdown lactose but does not. After talking to your lab technician about this you learn the MacConkey agar was not prepared correctly. Please discuss what method(s) can be performed and the results that would help confirm what the MacConkey agar would normally show.
Added by Kevin B.
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Step 1: Confirm that the MacConkey agar was not prepared correctly by checking the ingredients used and the preparation method. Show more…
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You inoculated a MacConkey agar plate with a gram-positive and a gram-negative bacterial strain. When you observe the plate after incubation, you notice that only the gram-negative bacteria formed colonies. In addition, you notice there is a color change in the media (red to yellow), indicating that this gram-negative bacterium is capable of lactose fermentation. What type of media is this? a) selective b) Differential c) All of the above 2. Fannie Hesse developed the idea of replacing gelatin with agar. What are the benefits of using agar over gelatin? a) Agar is cheap and a good solidifying agent b) Gelatin melts at a lower temperature (40 degrees Celsius), whereas agar does not. c) Agar is a great nutrient source for most bacteria. d) Answer A and B are correct 3. You have been instructed to measure an overnight culture for bacterial density. Which of the following methods would you use if you do not care about distinguishing dead from live bacterial cells? a) Membrane filtration method b) Turbidity c) Most probable number d) Plate count using the spread plate method e) Plate count using the pour plate method.
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