Question

10. E. coli MG1655 growing in LB at 37°C has a generation time of 45 min (0.75 h). You are given a culture of E. coli growing at 37°C in a new culture medium (NCM-1). The initial cell concentration is 1 × 10? cfu/ml. After 10 h, the cell concentration is 1 × 10? cfu/ml. How many generations of growth did the culture have during that 10 h period (to the nearest 0.1 generation)? What is the approximate generation time during that period (to the nearest 0.1 h)? Do the cells grow faster in LB or in NCM-1? [Note: log 2 = 0.301; show your work, as I will give partial credit for correctly set-up problems.]

          10. E. coli MG1655 growing in LB at 37°C has a generation time of 45 min (0.75 h). You are given a culture of E. coli growing at 37°C in a new culture medium (NCM-1). The initial cell concentration is 1 × 10? cfu/ml. After 10 h, the cell concentration is 1 × 10? cfu/ml. How many generations of growth did the culture have during that 10 h period (to the nearest 0.1 generation)? What is the approximate generation time during that period (to the nearest 0.1 h)? Do the cells grow faster in LB or in NCM-1? [Note: log 2 = 0.301; show your work, as I will give partial credit for correctly set-up problems.]

10. E. coli MG1655 growing in LB at 37°C has a generation time of 45 min (0.75 h). You are given a culture of E. coli growing at 37°C in a new culture medium (NCM-1). The initial cell concentration is 1 × 10? cfu/ml. After 10 h, the cell concentration is 1 × 10? cfu/ml. How many generations of growth did the culture have during that 10 h period (to the nearest 0.1 generation)? What is the approximate generation time during that period (to the nearest 0.1 h)? Do the cells grow faster in LB or in NCM-1? [Note: log 2 = 0.301; show your work, as I will give partial credit for correctly set-up problems.]

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